In this presentation, attendees will receive a detailed exploration of eDNA technology's evolution as a monitoring paradigm. Presentation will showcase its remarkable capabilities, focusing on how our cutting-edge eDNA/eRNA assay constitutes a leap forward in methodological precision and reliability. To illustrate the practical applications and effectiveness of this approach, case studies featuring Dreissenid mussels and larval fish will be presented, setting the stage for future research and conservation strategies that are both more informed and sustainable.